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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in many tumors, which ends up in chromosome instability, and therefore may perhaps add to tumorigenesis. This protein was observed to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two additional splice variants were being documented but in-depth nucleotide sequence info is not however out there.
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Blocking peptides are peptides that bind specially for the focus on antibody and block antibody binding. These peptide usually incorporates the epitope recognized via the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This manual outlines additional feasible optimization of cellular and immunoassay parameters to be certain the very best final results are...
Antibodies bound to the blocking peptide no more bind into the epitope on the target protein. By comparing the staining from your blocked antibody versus the antibody by itself, one can see which staining is particular.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin loved ones, whose members are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation styles which lead towards the temporal coordination of each and every mitotic event. Cyclin E types a complex with and functions for a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S changeover. This protein accumulates within the G1-S period boundary and is degraded as cells progress by S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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